Contact: Ms. Donna Wall, Staff Analyst, e-mail: dwall1@uky.edu Phone: (859) 323-6979 Fax: (859) 323-0381
Contact: Dr. Kuey-Chu Chen, Director; e-mail: kueyc@uky.edu; phone: (859) 323-6241
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nCounter System from NanoString Technologies
The nCounter Analysis System is a complete, fully automated system for the next generation of digital gene expression analysis. Through the use of a unique coding technology, direct counting of individual mRNA molecules is made possible across all levels of biological expression. The nCounter Analysis System requires only 100ng of total RNA or equivalent amounts of raw cell or blood lysate to study hundreds of genes. www.nanostring.com
Equipment:
1. Prep Station
Purifies hybridized samples and immobilizes them in a cartridge for data collection.
2. Digital Analyzer
Collects code counts for each barcode and provides a simple count for each gene and control. Accepts 6 cartridges with 12 samples per cartridge.
Reagents and Supplies:
1. nCounter Master Kit
All consumables and reagents required for sample processing on the Prep Station are provided in the nCounter Master Kit (including a cartridge holding 12 samples per cartridge) and come ready to load onto the deck of the robot. No reagent preparation or dilutions are required.
2. Custom CodeSet
The set of molecular barcodes specifically designed to target your genes of interest. In addition, a comprehensive mix of controls are added to each CodeSet to quantitate, QC, and normalize gene expression.
The custom CodeSets can be purchased through NanoString Technologies (www.nanostring.com). CONTACT: Chad Dodds (Regional Account Manager) at: cdodds@nanostring.com for CodeSet design.
For data quality assessment and analysis help, CONTACT: Kirsteen Maclean (Field Application Specialist) at kmaclean@nanostring.com.
Services and Fees:
Each investigator will need to purchase their own CodeSets. CodeSets are designed and purchased through NanoString Technologies by individual contact.
There are two options for hybridization:
A: Set up your own hybridization reactions in the Microarray lab.
B: Submit RNA samples to the Microarray lab to set up the hybridization.
The overnight hybridization needs to be carried out in the Microarray Facility in order to go directly to the Prep Station from incubation temperature. The Microarray facility is responsible for loading the hybridized samples onto the Prep Station and then transfer to the nCounter analyzer for data collection. Data will be emailed to the investigator.
The fees are charged based on per cartridge run (each cartridge holds up to 12 samples):
Hybridization setup - $20
Prep Station loading, Digital Analyzer usage and data collection - $65
nCounter Master Kit - $300 per kit (contains 1 cartridge)
Please contact the Microarray Facility for questions and scheduling (859)323-6979.