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Roche LightCycler 480 Real-Time PCR System and Robot Instruments for Genomic DNA and RNA Isolation

Location:  HSRB Room 346

Contact: Caixia Hou

Phone: 859-323-5249

Email: QRTPCR@uky.edu

Equipment, Reagents and Supplies

1. Roche LightCycler 480 (https://www.roche-applied-science.com/sis/rtpcr/htc/index.jsp)

2. Magnalyzer (https://www.roche-applied-science.com/sis/automated/compact/index.jsp)

3. Two MagnaPures (https://www.roche-applied-science.com/sis/automated/compact/index.jsp)

4. SYBR Green Master Mix

5. Probe Master Mix

6. Universal Probe Library (165 universal probes cover all transcripts of human, mouse, rat, primates, Drosophila, Arabidopsis and C. elegans). (https://www.roche-applied-science.com/sis/rtpcr/upl/index.jsp)

7. DNA and RNA isolation Kit for MagnaPure (https://www.roche-applied-  science.com/sis/automated/magna_lc/index.jsp)

Protocols are available.

Fee Services (subject to re-adjustment):

For Roche LightCycler 480, $13/run for 96-well plate or $19.50/run for 384-well plate

For MagnaPure, $8.25/run (up to 8 samples)

For MagnaLyser, $2.25/run (up to 16 samples)

For LightCycler 480 SYBR Green 1 Master(2X), $70/ml (enough for 100 reactions)

For LightCycler 480 Probe Master(2X), $65/ml (enough for 100 reactions)

For Magna Lyser Green Beads, $3/tube

For RNA Isolation Kit,  $11/sample

For Universal Probes, $12.5/10μl (enough for 50 reactions)

For plate and sealing foil, $8/plate and sealing foil

*If you need instruction in performing RT-PCR, we will assist with the first time use at a rate of $35/half hour.

*The price for external users is 5% higher than the price listed.