Daret K.
St. Clair, Ph.D.
Professor

Graduate Center for Toxicology

Phone:
(859) 257-3956

Fax:
(859) 323-1059

e-mail:
dstcl00@uky.edu

Dr. St. Clair's laboratory is investigating the fundamental mechanisms by which reactive oxygen species (ROS) and reactive nitrogen species (RNS) contribute to normal tissue injury and cancer formation. The ultimate goal is to develop novel strategies for intervention and improvement of treatment. Several separate, but related areas of research are in progress.

1. Transcriptional regulation of the human MnSOD gene expression

2 Redox-mediated mechanisms of tumor suppression

3. Antioxidant protective mechanisms against cardiac and neuronal injury.

Research Publications/Presentations

1. St. Clair, D.K., Wan, X.S., Oberley, T.D., Muse, K.E., St. Clair, W.H., Overproduction of mitochondrial superoxide dismutase suppresses radiation-induced neoplastic t ransformation. Molecular Carcinogenesis, 6: 238-242, 1992.

2. Wan, X.S., Devalaraja, M.N., and St. Clair, D.K., Molecular structure and characterization of the human MnSOD gene. DNA and Cell Biology, 13:1127-1136, 1994.

3. Safford, S.E., Oberley, T.D., Urano, M., and St. Clair, D.K., Overexpression of manganese superoxide dismutase suppresses metastasis of fibrosarcoma. Cancer Res, 54: 4261-4265, 1994.

4. Urano, M., Kuroda M., Oberley, T.D., and St. Clair, D.K., Transfection of manganese superoxide dismutase cDNA reduces tumor control radiation dose: Gene-Radiotherapy. Cancer Res. 55:2490-2493, 1995.

5. Yen, H-C, Oberley, T.D., Vichitbanda, S., Ho, Y-S, and St. Clair, D.K., The protective role of manganese superoxide dismutase against adriamycin-induced acute cardiac toxicity in transgenic mice. J. Clin. Invest. 98: 1253-1260, 1996.

6. Kiningham, K.K. and St. Clair, D.K. Overexpression of manganese superoxide dismutase selectively modulates the activity of jun-associated transcription factors in fibrosarcoma cells. Cancer Research 57: 5265-5271, 1997.

7. Majima, H.J. Oberley, T.D., Furakawa, K., Mattson, M.P., Yen, H-C, Szweda, L.I., and St. Clair, D.K. Prevention of mitochondrial injury by manganese superoxide dismutase reveals a primary mechanism for alkaline-induced cell death. J. Biol. Chem. 273: 8217-8224, 1998.

8. Yong, X., Krisnan, A., Wan, X.S., Majima, H., Yeh, C-C, Ludewig, G., Karsaksis, E.J, and St. Clair, D.K. Mutations in the promoter reveals a cause for the reduced expression of the human manganese superoxide dismutase gene in cancer cells. Oncogene. 18:93-102, 1999.

9. Kiningham, K.K., Oberley, T.D., Lin, S-M. Mattingly, C.A., and St. Clair, D.K. Overexpression of manganese superoxide dismutase (MnSOD) protects against mitochondrial-initiated poly(ADP-robose) polymerase-mediated cell death. Faseb J. 13:1601-1610, 1999.