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CSB FACILITIES / SERVICES:

    Proteomics Services 

    Cell Culture

    Crystallography

    SERCAT

    Computational Facilities

    Protein Characterization

        Chromatography 

        Fluorimetry

        Circular Dichroism

        Dynamic Light Scattering

        MicroCalorimetry

       TIRF Microscopy

CSB FACULTY

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Dept of Molecular and Cellular Biochemistry 

      

Spectroscopic Methods for Protein Structure

A dynamic laser light-scattering instrument (ProteinSolutions Dynapro99) is available for rapid determination of molecular weight and detection of aggregation. This determination is made from measuring molecular radius of multiple species in the sample. Aggregation can be detected if the monomer molecular weight is already known.  Aggregation is a hindrance to crystallization; therefore,  it is often useful to monitor this after making new constructs.

Dynapro 99 Light Scattering

A CD (Jasco 810 Spectropolarimeter) makes possible structural / conformational evaluation of proteins and peptides, as to alpha helix, proline II helix, and beta-sheet content.  Dr. Trevor Creamer is using this instrument for studying the structural properties of proteins susceptible to aggregation and misfolding, relevant to Huntington's disease.

  Jasco 810 CD

A MicroCal isothermal microcalorimeter (ITC) is available for determining thermodynamic characteristics of molecular binding interactions.

Microcal

      

Comments to Jack Schmidt, Last Modified: November 23, 2011
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