CSB FACILITIES / SERVICES:
Dynamic Light Scattering
Dept of Molecular and Cellular Biochemistry
CSB Facilities for
producing protein from bacteria, yeast, or insect cultures
High volumes for maximum production and purification of protein
|Several incubators are maintained at
temperatures optimized for yeast or insect cultures, as well as
bacteria. A sterile culture hood is available for mammalian / insect culture.
A 10-L fermenter (New Brunswick
BioFlow3000) allows growth at 37 C and
subsequent protein production upon induction of the incorporated plasmid
at a chosen (lower) temperature. This may permit protein to be produced
without degradation. Protein may be produced
over an extended time (overnight) with very high density of bacteria (or
yeast) due to very vigorous aeration (not attainable in shaker
Cell lysis is typically carried out with a standard French press. For high-volume lysis of paste from bacteria or yeast, a
microfluidics cell-cracking device is available. Cells are lysed by collision at high pressure.
Protein final purification by FPLC (Akta Explorer of GE Health Care) is also available, with programmable gradients, multiple wavelength detection and data storage, and fraction collectors..
Comments to Jack Schmidt, Last Modified:
November 23, 2011
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