CSB FACILITIES / SERVICES:
Dynamic Light Scattering
Dept of Molecular and Cellular Biochemistry
Instruments for Protein
Purification and Structural / Kinetic Studies
Chromatography and fluorimetry make possible studies of structural properties and enzymatic
activity of enzyme mutants.
Protein purification with automated fraction collector and computer
generated data is available using the gradient CSB Akta Explorer FPLC with multiple wavelength capability.
HPLC (Waters Breeze) which uses a programmable gradient and UV- vis dual wavelengrth detector can be used to evaluate peptides; in particular recently used to monitor slow kinetics of
enzyme-catalyzed hydrolysis by various mutant peptidases. This methodology is an
alternative to using fluorometric substrates.
A Perkin-Elmer LS55 spectrofluorometer
is used for studying a variety of molecular properties, including binding, RET (resonance energy transfer), molecular rotation (fluorescence polarization capability) as well as slow kinetic reactions using fluorometric substrates. A new phototube brings up the spectral domain into the near-infrared region e.g. with Cy 7 dyes, spectrally isolated from other fluorophores. The high sensitivity of this instrument is compatible
with very low concentrations. A new K2 fluorescence lifetime instrument has been acquired and is expected to be available in the near future.
Comments to Jack Schmidt, Last Modified:
November 23, 2011
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