LEICA TCS SP5 INVERTED CONFOCAL WITH MULTIPHOTON LASER (located in BBSRB)

Multiphoton laser- uv range for thick samples or samples in water

Visable light Lasers –argon with excitation lines 458nm, 476nm, 488 nm,  514nm,  and HeNe excitation lines 543nm and 633nm

Tranmitted light detector-Differential Interference Contrast (Nomarski) and brightfield

Objectives- 10x, 20x oil, 40x oil,  63x glycerin, 100x oil

Scan Formats – x, y, z, t, l        

Image Formats - 64 x 64 pixels to 8192 x 8192 pixels, in square or rectangular  formats

Electronic zoom

Optical Field Rotation- allows rotation of the image 200o

Spectral Imaging detection- includes five confocal channels, and allows freely adjustable positioning and bandwidth of the detection range

FLIM- (Fluorescence lifetime imaging) is used to determine cell parameters such as pH value, ion concentration or oxygen saturation and  protein interaction

Mark & Find- Defines the current position of the sample and recalls it later from a list of previously stored positions and can mark multiple cells for recording. It can be combined with time series and z scans so that dynamic processes can be recorded in a large sample at multiple positions over time, each with an independent z-stack.

Tile Scan- automatically assembles an image from multiple smaller images.   Large specimens that are bigger than the field of view can be viewed as a whole using tile scan.

Spectral Separation – useful for dyes whose emission ranges are close together or partially overlapping

Image Acquistion – download as TIFF images and save via  NeroStartSmart software to CDs, DVDs, USB flash drives, portable external hard drives