LEICA TSP SP5 CONFOCAL with ENVIRONMENTAL CHAMBER (in BBSRB)

FRET (fluorescence resonance energy transfer) Acceptor Bleach Wizard - a guide through the measurement process which gives quantitative data at the end. It is a technique that allows insight into interactions between proteins or molecules in proximities beyond light microscopic resolution.

FRAP (fluorescence recovery after photobleaching)- Employs irradiation of a fluorophore in a living sample with a short laser pulse to degrade it and thereby abolish fluorescence followed by time-resolved image recording for the sample. Measures dynamics within and between subcellular compartments, as well as changes in morphology

Tile Scan- automatically assembles an image from multiple smaller images. Large specimens that are bigger than the field of view can be viewed as a whole using tile scan.

Spectral Separation-useful for dyes whose emission ranges are close together or partially overlapping

Image Acquistion-download as TIFF images and save via NeroStartSmart software to CDs, DVDs, USB flash drives, portable external hard drives

Live Data Mode- Allows multiple jobs with different time sequences

Mark Find- Defines the current position of the sample and recalls it later from a list of previously stored positions and can mark multiple cells for recording. It can be combined with time series and z scans so that dynamic processes can be recorded in a large sample at multiple positions over time, each with an independent z-stack.

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