LEICA TSP SP5 CONFOCAL with ENVIRONMENTAL CHAMBER (in BBSRB)


PLEASE DOWNLOAD THE TRAINING DOCUMENT FOR THE SP5 INVERTED CONFOCAL MICROSCOPE (PDF, 372K)

Environmental Chamber- completely encloses the microscope,allowing control of temperature, humidity and CO2

Motorized stage- for precise movement in x,z and y. Can accommodate slides or culture dishes with coverslip inserts

Differential Interference Contrast Optics (Nomarski)- brightfield

Objectives- 10x,20x oil, 40x oil, 63x oil, 63x glycerin, 63x water, 100x oil

Lasers- 405 diode for uv, argon 488 , HeNe 543 , HeNe 594 , HeNe 633

AOBS (Acousto Optical Beam Splitter)- a switching valve for light, which is tunable to channel any laser line onto the sample and simultaneously transmits the emitted light to the detector, eliminating the need for dichroic mirrors, yielding improved image quality and low bleaching

Scan Formats- x, y, z, t, l

Image Formats - 64 x 64 pixels to 4096 x 4096 pixels, in square or rectangular formats

Electronic zoom

Tandem Scanner - Conventional scanner for classical morphology, and a resonant scanner for fast acquisition at 16,000 lines per second or 200 frames per second

Optical Field Rotation- allows rotation of the image 180 degrees


Spectral Imaging detection- includes five confocal channels, and allows freely adjustable positioning and bandwidth of the detection range

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