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Single Fiber Function
The Single Fiber Function Core is co-directed by Ken Campbell and Karyn Esser. It is located on the 6th floor of the Medical Sciences Building and occupies approximately 750 square feet. The facility is currently staffed by Ben Lawson, a senior laboratory technician, who works full-time on 'core projects'.
The Core has been established to help Center Members and other investigators to determine the functional properties of different types of skeletal and cardiac muscle cells. All of the experiments currently made in the Core are performed using chemically permeabilized preparations. Samples can be obtained from animal models or human biopsies and stored for several weeks at -20C before analysis.
The figure below shows a typical set of data records collected from a permeabilized rat soleus fiber immersed in solutions with different free Ca2+ concentrations. The table summarizes some of the parameters that can be measured from these records and some of the structural, posttranslational and isoform content changes that can alter them.
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Page last updated Wednesday, June 30, 2010