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Office: (859) 257-4456
Fax: (859) 257-8994
Lab: (859) 257-5553
Email: jzhan1@uky.edu
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Jiayou Zhang, Ph.D.
Associate Professor
Doctoral Studies: University of Texas, Austin.
Postdoctoral: University of Wisconsin-Madison.
Research Statement:
The World Health Organization estimates that there are about 42 million people infected with HIV in the world today, including almost 1 million people in the United States. Therapeutic intervention has remained focused on several anti-viral drugs which have been developed recently. However, these drugs are toxic and are not able to eradicate HIV replication permanently. The goal of our laboratory is to develop an HIV mutant that inhibits the replication of wild-type HIV. Dominant negative mutated HIVs are not only defective viruses in their own replication, but also greatly inhibit the replication of wild-type HIV when both viral types infect the same cell. We have isolated several HIV Gag-Pol mutants. When these mutants were placed in cells infected with wild-type HIV, the replication of wild-type HIV was reduced to less than 10-4. We are constructing a HIV vector which can be used to efficiently deliver those dominant negative mutants into cells. The final goal would be to use this therapy in vivo, so that patients infected with HIV have a better quality of life when compared with current therapies.
Selected Recent Publications:
Zhang, J. 2004. The host RNA polymerase II makes minimal contributions to retroviral frame-shift mutations. J. Gen. Virology 85: 2389-2395 [Abstract]
Burnett, S. H., E. J. Kershen, J. Zhang, L. Zeng, S. C. Straley, A. Kaplan, and D. Cohen. 2004. Conditional macrophage ablation in transgenic mice expressing a Fas-based suicide gene. J. Leukocyte Biology 75, 612-623 PDF
Li, T. and J. Zhang. 2004. Stable expression of three genes from a tricistronic retroviral vector containing a picornavirus and 9-nt cellular internal ribosome entry site elements. J. Virol. Meth. 115, 137-144 PDF
Li, T. and J. Zhang. 2002. Intramolecular recombinations of Moloney murine leukemia virus occur during minus-strand DNA synthesis. J. Virol. 76, 9614-9623 PDF
Zhang, J. and Y. Ma. 2001. Evidence for retroviral intramolecular recombination. J. Virol. 75: 6348-6358. PDF
Turchan J, Anderson C, Hauser K, Sun Q, Zhang J, Liu Y, Wise P, Kruman I, Mattson MP, Booze R, Maragos W, Nath A. 2001. Protection by estrogen of synergistic neurotoxicity of HIV proteins, methamphetamine and cocaine. BMC Neuroscience 2: 3 [Abstract]
Li, T. and J. Zhang. 2001. Retroviral Recombinations are Temperature dependent. J. Gen. Virol. 82: 1359-1364 PDF
Zhang, J. and C. M. Sapp. 2001. A novel retroviral vector that allows the magnetic selection of infected cells. J. Virol. Meth. 94: 1-6 PDF
Gurwell, J. A., A. Nath, Q. Sun, J. Zhang, K. M. Martin, Y. Chen. and K. F. Hauser. 2001. Syneristic neurotoxicity of opioids and human immunodeficiency virus-1 Tat protein in striatal neurons. Neuroscience. 102: 555-563 PDF
Li, T. and J. Zhang. 2000. Determination of the frequency of recombination between two identical sequences at a provirus. J. Virol. 74:7646-7650 PDF
Tang, L. Y and J. Zhang. 2000. The cellular mismatch repair system is able to repair mismatches within MLV retroviral double-stranded DNA at low frequency. Nucleic Acids Res. 28: 2302-2306 PDF
Zhang, J., L-Y. Tang, T. Li, Y. Ma and C. M. Sapp. 2000. Most retroviral recombinations occur during minus-strand DNA synthesis. J. Virol. 74: 2313-2322 PDF
Sapp, M. C., T. Li and J. Zhang. 1999. Systematic comparison of a color reporter gene and drug resistance genes for determination of retroviral titers. J. Bio. Sci. 6: 342-348 [Abstract]
Zhang, J. and C. M. Sapp. 1999. Recombination between two identical sequences within the same retroviral RNA molecule. J. Virol. 73: 5912-5917 PDF
Zhang, J., and H. M. Temin. 1993. Rate and mechanism of nonhomologous recombination during a single cycle of retroviral replication. Science 259: 234-238 [Abstract]
Zhang, J., W. Bargmann., and H. R. Bose, Jr. 1989. Rearrangement and diversification of immunoglobulin light chain genes in lymphoid cells transformed by reticuloendotheliosis virus. Mol. Cell Biol. 9: 4970-4976 [Abstract]
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