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Office: (859) 323-5598
Fax: (859) 257-8994
Lab: (859) 323-5751
Email: cmiller@uky.edu
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Craig S. Miller, D.M.D., M.S.
Professor
Doctoral Studies: University of Kentucky (College of Dentistry).
Postdoctoral: University of Texas Health Science Center, San Antonio.
Research Statement:
Our long-term goal is to understand how herpes simplex virus 1 and 2 (HSV) reactivate from a latent state. Following epithelial cell infection, HSV spreads to sensory neurons where it remains latent for the life of the host. Periodically, the virus reactivates from latency following stress and trauma. Our laboratory is interested in understanding the stress-associated regulatory processes that allow the virus to reactivate. Ongoing studies use neurally differentiated PC12 cells and animals as hosts of HSV-1 latent-like infection. Genes and proteins critical to reactivation are evaluated by subjecting quiescently-infected cells and latently infected animals to stimulatory agents associated with reactivation. Genes and proteins activated and/or repressed are identified using viral deletion mutants, Western, Southern and Northern blotting, reverse transcriptase polymerase chain reaction (RT-PCR), gene array, the chloramphenicol acetyl transferase (CAT) and luciferase assays, and enhanced green fluorescence. Other technologies used routinely are cell culture, direct plaque assay, mutant construction, immunocytochemistry, in situ hybridization, Northern blot analyses, electrophoretic mobility shift assay, the polymerase chain reaction, chromatin immunoprecipitation, and confocal and electron microscopy.
Selected Recent Publications:
Dawson, DR, Wang C, Danaher RJ, Lin Y, Kryscio RJ, Jacob RJ, Miller CS. Use of real time polymerase chain reaction to determine the prevalence and copy number of Epstein-Barr virus and cytomegalovirus DNA in subgingival plaque at individual healthy and periodontal disease sites. J Periodontol accepted February 26, 2009.
Schuster JL, Branscum A, Miller CS, Ebersole J, Al-Sabbargh M, Stevens J, Thomas MV. Variability in repeated measures of salivary analytes in healthy adults: A pilot study. J Periodontol, accepted February, 2009.
Dawson, DR, Wang C, Danaher RJ, Lin Y, Kryscio RJ, Jacob RJ, Miller CS. Salivary levels of EBV DNA correlate with subgingival levels, not severity of periodontitis. Oral Dis accepted May 13, 2009.
Frodge BD, Ebersole JE, Kryscio RJ, Thomas MV, Miller CS. Bone remodeling biomarkers of periodontal disease in saliva. J Periodontol 79:1913-9, 2008.
Miller CS, Armitage GC. Tetracycline-induced nephrotoxicity after dental treatment. JADA 140:56-60, 2009.
Combs DR, Reilly EA, Dawson III DR, Avdiushko SA, Danaher RJ, Miller CS. Detection of human cytomegalovirus in dental plaque by real-time PCR. Oral Surg Oral Med Oral Pathol Oral Radiol Endodon Epub September 19, 2008.
Danaher R.J., McGarrell B.S., Stromberg A.J., Miller C.S. (2008) Herpes Simplex Virus Type 1 Modulates Cellular Gene Expression During Quiescent Infection of Neuronal Cells. Archives of Virology Epub June 12, 2008.
Christodoulides N., Floriano P.N., Griffin M., Lennart A., Karri L., Ballard M., Mohanty S., Dharshan P., McDevitt J.T., Miller C.S., Ebersole J.L., King, Jr C.P., Langub M.C., Thomas M.V. (2007) Lab-on-a-chip methods for point-of-care measurements of salivary biomarkers of periodontitis. Ann NY Acad Sci 1098:411-28.
Miller C.S. and Danaher R.J. (2007) Asymptomatic shedding of herpes simplex virus in the oral cavity. Oral Surg Oral Med Oral Pathol Oral Radiol Endodon 105:43-50.
Brennan M.T., Wynn R.L., Miller C.S. (2007) Aspirin and bleeding in dentistry: An update and recommendations. Oral Surg Oral Med Oral Pathol Oral Radiol Endodon 104:316-323.
Miller C.S., Danaher R.J., and Jacob R.J. (2006) ICP0 is not required for efficient stress-induced reactivation of herpes simplex virus type 1 from cultured quiescently infected neuronal cells J Virol 80: 3360-3368.
Berger J.R., Miller C.S., Mootoor Y., Avdiushko S.A., Kryscio R.J., Zhu H. (2006) JC virus detection in bodily fluids: clues to transmission. Clin Infect Dis 43:e9-12.
Albuquerque R.J.C., de Leeuw R., Carlson C.R., Okeson J.P., Miller C.S., Andersen A.H. (2006) Cerebral activation during thermal stimulation of patients who have burning mouth disorder: An fMRI study. Pain 122: 223-234.
Miller C.S., Berger J.R., Mootoor Y., Avdiushko S.A., Zhu H., and Kryscio R.J. (2006) High prevalence of multiple human herpesviruses in saliva from human immunodeficiency virus-infected persons in the era of highly active antiretroviral therapy (HAART). J Clin Micro 44: 2409-2415.
Danaher R.J., Jacob R.J., Miller C.S. (2006) Reactivation from quiescence does not coincide with a global induction of herpes simplex virus type 1 transactivators. Virus Genes33:163-167.
Danaher R.J., Jacob R.J., Steiner M.R., Allen W.R., Hill J.M., Miller C.S. (2005) Histone deacetylase inhibitors induce herpes simplex virus type 1 reactivation in a latency associated transcript (LAT)-independent manner. J. NeuroVirol11: 306-317.
Miller C.S. (2005) Pleiotropic mechanisms of virus survival and persistence. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 100 (Suppl 2): S27-S36.
Miller C.S., S.A. Avdiushko, R.A. Kryscio, R.J. Danaher, and R.J. Jacob (2005) Effect of prophylactic valacyclovir on the presence of human herpesviruses DNA in saliva of healthy individuals following dental treatment. J Clin Microbiol 43: 2173–2180.
Rhodus R.L., Ho V., Miller C.S., Myers S., Ondrey F. (2005) NF-k B dependent cytokine levels in saliva of patients with oral preneoplastic lesions and oral squamous cell carcinoma. J Cancer Detect Prev 29: 42-45.
Christodoulides N., Mohanty S., Miller C.S., Langub M.C., Floriano P.N., Dharshan P., Ali M., Bernard B., Romanovicz D., Anslyn E., Fox P.C., McDevitt J.T. (2005) Application of microchip assay system for the measurement of C-reactive protein in human saliva. Lab on a Chip 5: (3) 261-269.
Miller, C.S., Cunningham L.L., Lindroth J.E., Avdiushko S.A. (2004) The efficacy of valacyclovir in preventing recurrent herpes simplex virus infections associated with dental procedures. J Am Dent Assoc 135: 1311-1318.
Miller, C.S., Bhattacharjee P.S., Higaki S., Jacob R.J., Danaher R.J., Thompson H.W., Hill J.M. (2003) Herpesvirus quiescence (QIF) in neuronal cells VI: Correlative analysis demonstrates usefulness of QIF-PC12 cells to examine HSV-1 latency and reactivation and deregulated LAT ORF expression. Curr Eye Research 26(3-4): 239-248.
Rhodus N.L., Carlson C.R., Miller C.S. Burning mouth (syndrome) disorder. Quintessence Int 34:587-593, 2003.
Danaher, R.J., Jacob R.J., Miller C.S. (2003) Herpesvirus quiescence in neuronal cells V: Forskolin-responsiveness of the herpes simplex virus type 1 a0 promoter and contribution of the putative CRE element. J NeuroVirol9: 489-497.
Silverman, S., Miller C.S. (2003) Diagnosis and treatment of viral infections of the maxillofacial region. Oral and Maxillofac Surg Clinics 15(1): 79-89.
Danaher, R.J., Savells-Arb A.D., Black Jr S.A., Jacob R.J., Miller C.S. (2001) Herpesvirus quiescence in neuronal cells IV: reactivation induced by pituitary adenylate cyclase activating polypeptide (PACAP) involves the protein kinase A pathway. J NeuroVirol 7: 163-168.
Miller, C.S., Johnstone B.M. (2001) Human papillomavirus infection as a risk factor for oral squamous cell carcinoma: a meta-analysis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 91: 622-635.
Danaher, R.J., Jacob R.J., Miller C.S. (2000) Herpesvirus quiescence in neuronal cells: antiviral conditions not required to establish and maintain HSV-2 quiescence. J NeuroVirol6: 296-302.
Danaher R.J., Jacob R.J., Chorak M.D., Freeman C.S., Miller C.S. (1999) Heat stress induces reactivation of herpes simplex virus type 1 infection from quiescently infected neurally differentiated PC12 cells. J NeuroVirol 5: 374-383.
Danaher R.J., Jacob R.J., Miller C.S. (1999) Establishment of a quiescent herpes simplex virus type 1 infection in neurally differentiated PC12 cells. J NeuroVirol 5: 258-67.
Miller C.S., Danaher R.J., Jacob R.J. (1998) Molecular aspects of herpes simplex virus type 1 latency, reactivation and recurrence. Crit Rev Oral Biol and Oral Med 9:541-62.
Miller C.S., White D.K. (1996) Human papillomavirus expression in oral mucosa, premalignant conditions and squamous cell carcinoma: a retrospective review of the literature. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 82: 57-68.
Miller C.S. (1996) Viral infections in the immunocompetent patient. Dent Clin N Am 14: 225-241.
Miller C.S, Zeuss M.S., White D.K. (1994) Detection of HPV DNA in oral carcinoma using polymerase chain reaction and in situ hybridization. Oral Surg Oral Med Oral Pathol 77: 480-486.
Miller C.S. (1994) Herpes simplex virus and human papillomavirus infections of the oral cavity. Sem Dermatol 13: 108-117.
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