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Office: (859) 323-5598
Fax: (859) 257-8994
Lab: (859) 323-5751
Email: cmiller@uky.edu
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Craig S. Miller, D.M.D., M.S.
Professor
Doctoral Studies: University of Kentucky (College of Dentistry).
Postdoctoral: University of Texas Health Science Center, San Antonio.
Research Statement:
Our long-term goal is to understand how herpes simplex virus 1 and 2 (HSV) reactivate from a latent state. Following epithelial cell infection, HSV spreads to sensory neurons where it remains latent for the life of the host. Periodically, the virus reactivates from latency following stress and trauma. Our laboratory is interested in understanding the stress-associated regulatory processes that allow the virus to reactivate. Ongoing studies use neurally differentiated PC12 cells and animals as hosts of HSV-1 latent-like infection. Genes and proteins critical to reactivation are evaluated by subjecting quiescently-infected cells and latently infected animals to stimulatory agents associated with reactivation. Genes and proteins activated and/or repressed are identified using viral deletion mutants, Western, Southern and Northern blotting, reverse transcriptase polymerase chain reaction (RT-PCR), gene array, the chloramphenicol acetyl transferase (CAT) and luciferase assays, and enhanced green fluorescence. Other technologies used routinely are cell culture, direct plaque assay, mutant construction, immunocytochemistry, in situ hybridization, Northern blot analyses, electrophoretic mobility shift assay, the polymerase chain reaction, chromatin immunoprecipitation, and confocal and electron microscopy.
Grants:
Center for the Biologic Basis of Oral/Systemic Diseases. National Institutes of Health, Center of Biomedical Research Excellence (COBRE) 2P20RR020145-06 “Gene Therapy for Orofacial Pain”, 2009-2014
“Sites of JC Virus Latency and Genotypic Differences Underlying Tropism” Biogen, 2010-2012
Development of a Point of Care Immunoassay Test Strip for the Diagnosis of Periodontal Disease Innovation in Oral Care Awards, IADR and GlaxoSmithKline (GSK), 2010-2012
Evaluation of BTL-tml for Recurrent Oral Herpes Simplex Virus Infections” BeechTree Labs, 2011-2012.
Selected Recent Publications:
Danaher RJ, Wang C, Dai J, Mumper RJ, Miller CS. Antiviral effects of blackberry extract against herpes simplex virus type 1. Oral Surg Oral Med Oral Pathol Oral Radiol Endodon 112:e31-e35, 2011.
Sexton M, Lin Y, Kryscio RJ, Dawson DR, Ebersole JL, Miller CS. Salivary biomarkers of periodontal disease in response to treatment. J Clin Periodontol 38(5): 434-441, 2011.
Syrjanen S, Lodi G, von Bultzingslowen I, Aliko A, Arduino P, Campisi G, Challacombe S, Ficarra G, Flaitz C, Mei ZH, Maeda H, Miller C, Jontell M. Human papillomaviruses in oral carcinoma and oral potentially malignant disorders: a systemic review. Oral Dis 17 (Suppl 1):58-72, 2011.
Mirrielees J, Crofford LJ, Lin Y, Kryscio RJ, Dawson DR, Ebersole JL, Miller CS. Rheumatoid arthritis and salivary biomarkers of periodontal disease. J Clin Periodontol 37(12):1068-1074, 2010.
Danaher RJ, Kaetzel CS, Greenberg RN, Wang C, Miller CS. HIV protease inhibitors alter innate immune response signaling to double stranded RNA in oral epithelial cells: implications for immune reconstitution inflammatory syndrome? AIDS 24(16):2587-2590, 2010.
Yeh C-K, Christodoulides MJ, Floriano PN, Miller CS, Ebersole J, Weigum SE McDevitt J, Redding SW. Current development of saliva/oral fluid-based diagnostics. Texas Dent J 127(7):651-661, 2010.
Miller CS, Foley JD, Bailey AL, Campbell CL, Humphries RL, Christodoulides N, Floriano PN, Simmons G, Bhagwandin B, Jacobson JW, Ebersole JL, McDevitt JT. Current developments in salivary diagnostics. Biomark Med 4(1): 171-189, 2010.
Danaher RJ, Wang C, Roland AT, Kaetzel CS, Greenberg RN, Miller CS. HIV protease inhibitors block oral epithelial cell DNA synthesis. Arch Oral Biol 55:95-100, 2010, December 23, 2009 epub.
Wang CC, Yepes, LC, Danaher RJ, Berger JR, Mootoor Y, Kryscio RJ, Miller CS. Low prevalence of varicella zoster virus and herpes simplex virus type 2 in saliva from human immunodeficiency virus-infected persons in the era of highly active antiretroviral therapy. Oral Surg Oral Surg Oral Med Oral Pathol Oral Radiol Endodon 109: 232-237, 2010.
Dawson, DR, Wang C, Danaher RJ, Lin Y, Kryscio RJ, Jacob RJ, Miller CS. Salivary levels of EBV DNA correlate with subgingival levels, not severity of periodontitis. Oral Dis 15:554-559, 2009; June 12, 2009 epub.
Thomas MV, Branscum A, Miller CS, Ebersole J, Al-Sabbargh M, Schuster JL. Variability in repeated measures of salivary analytes in healthy adults: A pilot study. J Periodontol 80(7):1146-1153, 2009.
Dawson, DR, Wang C, Danaher RJ, Lin Y, Kryscio RJ, Jacob RJ, Miller CS. Use of real time polymerase chain reaction to determine the prevalence and copy number of Epstein-Barr virus and cytomegalovirus DNA in subgingival plaque at individual healthy and periodontal disease sites. J Periodontol 80(7):1133-1140, 2009.
Floriano PN, Christodoulides N, Miller CS, Ebersole JL, Spertus J, Rose BG, Kinane DF, Novak MJ, Acosta S, Mohanty S, Dharshan P, Yeh C-K, Redding S, Furmaga W, McDevitt JT. Use of saliva-based nano-bio-chip tests for acute myocardial infarction at the point-of-care: a feasibility study. Clin Chem 55(8):1530-1538, 2009; epub June 25, 2009.
Frodge BD, Ebersole JE, Kryscio RJ, Thomas MV, Miller CS. Bone remodeling biomarkers of periodontal disease in saliva. J Periodontol 79(10):1913-9, 2008.
Combs DR, Reilly EA, Dawson III DR, Avdiushko SA, Danaher RJ, Miller CS. Detection of human cytomegalovirus in dental plaque by real-time PCR. Oral Surg Oral Med Oral Pathol Oral Radiol Endodon 2008; 106:840-44, ePub September 19, 2008.
Danaher RJ, McGarrell BS, Stromberg AJ, Miller CS. Herpes simplex virus type 1 modulates cellular gene expression during quiescent infection of neuronal cells. Archives of Virology. 2008;153(7):1335-45. Epub ahead of print June 12, 2008.
Dawson, DR, Wang C, Danaher RJ, Lin Y, Kryscio RJ, Jacob RJ, Miller CS. Use of real time polymerase chain reaction to determine the prevalence and copy number of Epstein-Barr virus and cytomegalovirus DNA in subgingival plaque at individual healthy and periodontal disease sites. J Periodontol accepted February 26, 2009.
Schuster JL, Branscum A, Miller CS, Ebersole J, Al-Sabbargh M, Stevens J, Thomas MV. Variability in repeated measures of salivary analytes in healthy adults: A pilot study. J Periodontol, accepted February, 2009.
Dawson, DR, Wang C, Danaher RJ, Lin Y, Kryscio RJ, Jacob RJ, Miller CS. Salivary levels of EBV DNA correlate with subgingival levels, not severity of periodontitis. Oral Dis accepted May 13, 2009.
Frodge BD, Ebersole JE, Kryscio RJ, Thomas MV, Miller CS. Bone remodeling biomarkers of periodontal disease in saliva. J Periodontol 79:1913-9, 2008.
Miller CS, Armitage GC. Tetracycline-induced nephrotoxicity after dental treatment. JADA 140:56-60, 2009.
Combs DR, Reilly EA, Dawson III DR, Avdiushko SA, Danaher RJ, Miller CS. Detection of human cytomegalovirus in dental plaque by real-time PCR. Oral Surg Oral Med Oral Pathol Oral Radiol Endodon Epub September 19, 2008.
Danaher R.J., McGarrell B.S., Stromberg A.J., Miller C.S. (2008) Herpes Simplex Virus Type 1 Modulates Cellular Gene Expression During Quiescent Infection of Neuronal Cells. Archives of Virology Epub June 12, 2008.
Christodoulides N., Floriano P.N., Griffin M., Lennart A., Karri L., Ballard M., Mohanty S., Dharshan P., McDevitt J.T., Miller C.S., Ebersole J.L., King, Jr C.P., Langub M.C., Thomas M.V. (2007) Lab-on-a-chip methods for point-of-care measurements of salivary biomarkers of periodontitis. Ann NY Acad Sci 1098:411-28.
Miller C.S. and Danaher R.J. (2007) Asymptomatic shedding of herpes simplex virus in the oral cavity. Oral Surg Oral Med Oral Pathol Oral Radiol Endodon 105:43-50.
Brennan M.T., Wynn R.L., Miller C.S. (2007) Aspirin and bleeding in dentistry: An update and recommendations. Oral Surg Oral Med Oral Pathol Oral Radiol Endodon 104:316-323.
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