Office: (859) 323-6207
Fax: (859) 257-8994
Lab: (859) 257-1006
Robert J. Jacob, Ph.D.
Doctoral Studies: Syracuse University.
Postdoctoral: University of Chicago.
Course Director: MI822 - Immunity, Infection and Disease
Awards: Master Educator Awards (5 Distinguished Areas), Liaison to the ADEA National Committee on Curriculum Change and Innovation
Opportunities to investigate the pathogenesis of herpesvirus infections are available in this molecular virology laboratory. Research is focused on understanding how herpesviruses replicate in susceptible cells. The delineation of events leading to the maturation of infectious virus is important because it will contribute to understanding the molecular pathogenesis of the disease. In one aspect of the work, research is focused on understanding cells infected with either human or equine herpesviruses and the events leading to naturally aborted and persistent infections. Understanding the aborted productive and persistently infected cell is important to virologists studying viral latency, as well as cancer. In a second aspect, research is focused on how the chromosomes of human herpesviruses replicate themselves during the first round of DNA replication. This point in the maturation of human herpesviruses appears to be pivotal in establishment and reactivation from the latent infection, and a focal point for targeting anti-herpetic drugs used in the treatment of the disease. Contemporary protocols used in protein and nucleic acid chemistry, such as cloning, RT-PCR, gel electrophoresis, restriction enzyme mapping, electron microscopy (see image), and antibody identification are currently used in this research. A variety of herpesvirus strains and mutants are also available.
In addition to these opportunities, the laboratory is interested in the physical properties of macromolecules and polymers. This interest focuses on how their properties contribute to the morphology and ultrastructure of biological and synthetic structures. Again, contemporary techniques such as low voltage, ultrahigh resolution electron microscopy and flourescent microscopy (enhanced green fluorescence) are utilized.
Selected Recent Publications:
Chunmei Wang, Dolphus R. Dawson III, Robert J. Danaher, Yushun Lin, M.S., Richard J. Kryscio, Robert J. Jacob,and Craig S. Miller, Relationship of Salivary Epstein-Barr Virus and Cytomegalovirus with Periodontal Disease, (2009) Submitted to Archives of Oral Biology
Jacob, R. J., Getting Started on becoming a Life Long Learner: A Primer on Comprehension for Medical and Dental Students, (2007) The Advisor 27:11
Baines, J. D.,E. Willis, R.J.Jacob, J. Pennington, and B. Roizman. Glycoprotein M of Herpes Simplex 1 is incorporated into virions during budding at the inner nuclear membrane. J. Virol. (2007) 81:800-812 [Abstract]
Miller,C.S.,R.J.Danaher,and R.J.Jacob, ICP-O is not Required for Efficient Stress Induced Reactivation of Herpes Simplex Virus Type 1 from Cultured Quiescently Infected Neuronal Cells. J. Virol.(2006) 80:3360-3368 [Abstract]
Danaher,R.J.,,R.J.Jacob,and Craig S. Miller, Reactivation from Quiescence Does Not Coincide with a Global Induction of Herpes Simplex Virus Type 1 Transactivators. Virus Genes. (2006) 33:163-167
Stratton,T.D.,D.B.Witzke,M.J.Freund,M.T.Wilson,and R.J.Jacob.,Validating Dental and Medical Students’evaluations of Faculty Teaching in an Integrated,Multi-Instructor Course.(2005),J. Dent.Ed.69:663-670
Miller,C.S.,S.A.Avdiushko,R.J.Kryscio,R.J.Danaher,R.J.Jacob Effect of Prophylactic Valacyclovir on the Presence of Human Herpesvirus DNA in Saliva of Healthy Individuals following Dental Treatment.(2005),J.Clin.Micro.43:2173-2180 [PDF]
Danaher,R.J.,R.J.Jacob,M.S.Steiner,W.R.Allen,J.Hill and C.Miller:Histone Deacetylase Inhibitors Induce HSV-1 Reactivation from Quiescently Infected PC-12 Cells Independent of the Latency-Associated Transcript.(2005) J.Neuro.Virol.11:306-317
Miller,C.S.,P.S.Bhattacharjee, S.Higaki,R.J.Jacob, R.J.Danaher, H.W.Thompson and J.M. Hill, Herpes virus Quiescence (QIF)in Neuronal Cells VI: Correlative Analysis Demonstrates Usefulness of QIF-PC12 Cells to Examine HSV-1 Latency, Reactivation and Genes Implicated in its Regulation.(2003) Curr.Eye,26:239-248
Lopez, P.,R.J.Jacob and B. Roizman. Overexpression of Promyelocytic Leukemia Protein precludes the dispersal of ND10 structures and has no effect on accumulation of infectious Herpes Simplex Virus 1 or its proteins. J.Virol.76:9355-9367, 2002 [Abstract]
Danaher,R.J.,,R.J.Jacob, and Craig S. Miller, Herpes virus Quiescence in Neuronal Cells:Forskolin-responsiveness of Herpes Simplex virus type 1 alpha”0” promoter and contribution of the putative CRE element.(2003) J.Neuro Virol.9:1-9.
Danaher, R.J., A.D.Savells-Arbs, S.A. Black Jr.,R.J. Jacob and C.S. Miller. Herpesvirus Quiescence in Neuronal Cells: Virus activation induced by pituitary adenylate cyclase-activating polypeptide (PACAP) involes the protein kinase A pathway. J.Neuro.Virol., 7:1-6, 2001
Danaher, R.J., R.J. Jacob and C.S. Miller. Herpesvirus Quiescence in Neuronal Cells: Antiviral conditions are not required to establish and maintain HSV-2 Quiescence. J.Neuro.Virol., 6:296-302, 2000
Danaher,R.J. , R.J.Jacob, and C.S.Miller Heat stress activates Herpes Simplex Virus Type 1 from Quiescently infected neurally differentiated PC-12 cells. J.Neuro. Virol. 5:374-383, 1999
Danaher,R.J. , R.J.Jacob, and C.S.Miller Estabnlishment of a Quiescent Herpes Simplex Virus Type 1 Infection in neurally differentiated PC-12 cells. J.Neuro. Virol. 5:258-267, 1999
Leavell, Jr., U.W., and Jacob, R.J. , Contagious Pustular Dermatitis, Contagious Echyma:Orf Virus Infection. In 5th Edition "Dermatology in General Medicine," Eds. T.B. Fitzpatrick, A.Z. Eiser, K.Wolf, I.M. Freedbery and K.F. Austen, McGraw-Hill Publishing Co., 1999
Miller,C.S., Danaher,R.J., and Jacob,R.J., Molecular Aspects of Herpes Simplex Virus I Latency, Reactivation and Recurrence. Critical Reviews in Oral Biology & Medicine,1998
Baines,J.D., Jacob, R.J., Simmerman, L., and Roizman, B. The Herpes Simplex Virus 1UL11 proteins are associated with cytoplasmic and nuclear membranes and with nuclear bodies of infected cells. J.Virology, 69:825-833,1995 [PDF]
Jacob, R.J., Price, R., Bouchesy, D., Davis, T. and Borchelt, J. Temperature sensitivity of equine herpes virus isolates: A brief review. S.A.A.S. Bulletin: Biochemistry and Biotechnology, 3:124-128, 1990