Flow Cytometry Core Facility
Jennifer Strange
Greg Bauman
(859)323-6859
Fax: (859)257-8994
Don Cohen (Director)
dcohen@uky.edu
(859)323-5131
This facility provides state-of-the-art analysis and sorting of single-cell populations for research. The facility offers a Cytomation MoFlo cell sorter with three lasers and high-speed cell sorting capability, and a Becton-Dickinson FacsCalibur cell analyzer and a Miltenyi-Biotech autoMACS separator for magnetic cell sorting. Researchers can schedule to have their samples analyzed/sorted for membrane, cytoplasmic and nuclear antigen expression, light-scattering properties, DNA content, cell cycles, and apoptosis, as well as measure intracellular biochemical changes such as calcium flux and pH. Researchers must schedule time at least 72 hours in advance.
