Office phone: (859)323-5207
Fax: (859)257-8994
Department of Microbiology, Immunology and Molecular Genetics
Jeffrey N. Davidson, Ph.D. |
Professor |
Doctoral studies: Harvard University. Postdoctoral: Eleanor Roosevelt Institute for Cancer Research. |
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Office phone: (859)323-5207 |
Selected publications |
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| Research statement: My laboratory is interested in studying the regulation, expression and organization of genes and proteins in mammalian cells. Experiments primarily involve the multienzymatic CAD protein, a protein that carries the first three enzymes of pyrimidine biosynthesis. To dissect the organization and regulation of this important protein, a variety of recombinant DNA, biochemical, and somatic cell genetic methods are being employed. For example, the activity of the first enzyme is affected by phosphorylation at a serine residue in hamster cells. When this residue is replaced by a glutamic acid, the enzyme behaves as if it has a constitutively phosphorylated serine. Another example involves a Drosophila Suppressor of Black mutant. The residue altered in the fly CAD protein has been identified and shown to reduce the first enzyme's response to feedback inhibition by UTP. Studies also include the expression of hamster protein in E. coli and hamster cells deficient in CAD; the use of single-step protein purification, site-directed mutagenesis and transgenic flies. Soon, experiments will be extended to yeast and transgenic mice. | |
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