< backDaugherty Lab Protocols

1. Angiotensin infusion

• Preparation of Alzet Pumps
• Implantation of Alzet Pumps
• AngII pump calculations-28days

2. Quantitation of mouse atherosclerotic lesions

• Figure 1
• Figure 2
• Figure 3

3. In Vivo

• Kent Coda 8
• Kent Coda 8 Video
• In vivo ultrasound
• Bone marrow

4. PCR proctols - all for mouse

• iCycler PCR manual
• ACE
• ACE2 (1)
• ACE2 (2)
• ACE2 (3)
• Angiotensinogen
• Angiotensinogen Deltaflox
• Angiotensinogen LoxP1
• ApoE
• AT1a
• AT1a receptor flox
• AT2
• CCR-2
• CRE & IL2
• Interleukin-4
• Interferon-gamma
• LDL receptor
• MMP-2
• MMP-9
• MyD88
• Osteopetrotic mice
• PPAR
• RAG1
• RAP x LDLR
• RAP PCR
• Renin
• Transgelin-Cre
• TEK-Cre
• uPA
• UPAR