CSB Facilities for producing protein from bacteria, yeast, or insect cultures
High volumes for maximum production and purification of protein

A 10-L fermenter (New Brunswick BioFlow3000) allows growth at 37 C and subsequent protein production upon induction of the incorporated plasmid at a chosen (lower) temperature.  This may permit protein to be produced without degradation. Protein may be produced over an extended time (overnight) with very high density of bacteria (or yeast) due to very vigorous aeration (not attainable in shaker flasks). 

For high-volume (> 70 mL) lysis of paste from bacteria or yeast, a Microfluidics International cell-cracking device is available.  A special pump produces 20,000 psi pressure from a 50 psi compressed air regulator.  Cells are lysed by collision. 

Protein final purification by FPLC (Akta Explorer of Amersham / Pharmacia Biotech) is also available.