University of Kentucky Proteomics Core Facility

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Services Presently Available

  • Gel spot cutting

  • Digestion of proteins in gel or solution

  • Identification of peptides by MS and MS/MS analysis

  • Database searching for protein identification

  • 2D gel electrophoresis and staining  (available through Kendrick labs)

  • Gel imaging

  • Biomarker discovery using Ciphergen SELDI system

  • Phosphopeptide enrichment and identification

  • 2D DIGE (available through Applied Biomics)

2-D DIGE (2-D Fluorescent Difference Gel Electrophoresis) is a method of differential protein expression detection and quantitation at the protein level.  Up to three samples are labeled with one of three different fluorescent dyes, combined and separated on the same 2-D gel.  Like proteins migrate together, and the amount of a particular protein originating from each original sample is determined by scanning the gel at different wavelengths for fluorescent dye quantitation.  For additional information on 2-D DIGE go to: http://www1.gelifesciences.com/aptrix/upp01077.nsf/Content/2d_electrophoresis~new_to_2d_dige

Service Charges -effective July 1, 2009

Chemical or enzymatic fragmentation of proteins supplied as a gel slice, in solution or on a membrane:  $162.50 for the first sample, $39 for each additional sample supplied at the same time  

LC MS/MS mass spectrometric analysis of peptides and sequence analysis of peptide fragments including MASCOT database sequence similarity search:      $131.25

MALDI TOF/TOF mass spectrometric analysis of peptides and sequence analysis of peptide fragments including MASCOT database sequence similarity search:   $88.50

Chemical protein modification:  $45/h

Phosphopeptide identification:   $45/h

Technical support:  $61.75/h

Protein mass measurement:  $59

Ciphergen targets: $85

MS analysis of Ciphergen targets:  $9.78 per spot

Ciphergen target preparation/data analysis:   $50/h

2D gel electrophoresis:  :  Kendrick labs will provide individualized quotes for each investigator and can help plan an analysis.  Prices begin at $175 for a 16 x 13 cm Coomassie blue stained gel.

Gel imaging:   $25  

2-D Fluorescent Difference Gel Electrophoresis (2D-DIGE):  Applied Biomics will help you design your experiment.  Prices begin at $1200 for 2 CyDye labeling. 

Services Available in the Near Future

  • Quantitative proteomics using iTRAQ labeling system and/or other methods;

  • Biomarker discovery using differential expression analysis;

  • de novo sequence analysis of peptides using MALDI TOF/TOF and peptide labeling techniques