AAA PPG Mouse Core

Personnel

Abstract

Protocols

Representative Publications

Personnel

 

Debra Rateri

Debra L. Rateri, B.S.
Core Director

 

 

 

 

 

 

 

 

 

Jessica Moorleghen, B.S.
 

 

 

Abstract

The purpose of this Core is to provide a central location for the supply of mice and services related to mouse care, maintenance, genotyping, and measurement.  Specifically this Core will offer the following services:

1.  Managing the mouse colony that includes the oversight of the generation, maintenance, and supply of single and compound genetically altered mice.
2.  Perform bone marrow transplantation.
3.  Measure arterial blood pressure in a standardized manner.
4.  Prepare and implant Alzet pumps for the infusion of AngII or saline.
5.  Harvest DNA by tail clipping for the purpose of genotyping.
6.  In vivo ultrasound.  Non-invasively quantify lumen diameter during the evolution of AAAs.

Centralization of these facilities will permit an efficient use of resources for the Program, coupled with optimal quality control and standardization across Projects. 

 

Protocols

Genotyping:  
  • APOE (PDF)
  • AT1a receptor  (PDF)
  • Transgelin Cre  (PDF)
  • AT1a receptor flox  (PDF)
  • LDL receptor  (PDF)
  • PCR  (PDF) 
  • RAP x LDLR (PDF)
  • TEK-Cre (PDF)
  • uPA  (PDF)
  • UPAR (PDF)
     

Bone marrow transplantation:  (PDF)

Systolic blood pressure measurement:  (PDF)

Alzet pump implantation:  (PDF)

DNA harvest:  Mice at 3 weeks of age will be ear marked, numbered, and tail clipped. Genomic DNA will be isolated using Qiagens DNeasy Tissue Kits. The isolated DNA will be given to each Investigators laboratory for PCR genotype screening.

In vivo ultrasound:  (PDF)

Representative Publications

1.  Daugherty, A. , M. W. Manning, and L.A. Cassis. (2000) Angiotensin II promotes atherosclerotic lesions and aneurysms in apolipoprotein E deficient mice. Journal of Clinical Investigation. 105:1605-1612.

2.  Daugherty, A. and L.A. Cassis (2002) Mechanisms of arterial aneurysm formation. Current Atherosclerosis Reports. 4:222-227.

3.  Daugherty, A. and S.C. Whitman. (2003) Use of the mouse for studying atherosclerosis. The Transgenic Mouse: Methods and Protocols. pg 293-309. Ed. M. Hofker and J. van Deursen. Humana Press, Totowa, New Jersey.

4.  Daugherty, A. and D.L. Rateri. (2005) Development of experimental designs for atherosclerosis studies in mice. Methods. 36: 129-138.

5.  Daugherty, A. and D.L. Rateri (2006) Hyperlipidemia-induced atherosclerosis. A Handbook of Mouse Models for Cardiovascular Diseases. pg. 53-66. Ed Q. Xu, Wiley, London.

6.  Barisione, C, D.L. Howatt, J. Moorleghen, D.L. Rateri, and A. Daugherty. (2006) Rapid dilation of the abdominal aorta during infusion of angiotensin II detected noninvasively by high frequency ultrasound. Journal of Vascular Surgery. In press.

7.  Wang, Y.X., L.A. Cassis, and A. Daugherty. (2006). Angiotensin II-induced abdominal aortic aneurysms. A Handbook of Mouse Models for Cardiovascular Diseases. pg 125-146. Ed Q. Xu, Wiley, London.

8.  Lu, H., D.L. Rateri, and A. Daugherty (2007) Immunostaining of mouse atherosclerotic lesions. Vascular Biology Protocols. Eds. Sreejayan and J. Ren. Human Press, Totowa, New Jersey. In press.

 

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Comments: Darin Cecil | Last Modified: 10/13/2010
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